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KMID : 1134820090380101466
Journal of the Korean Society of Food Science and Nutrition
2009 Volume.38 No. 10 p.1466 ~ p.1470
Antimicrobial Effect of Edible Pullulan Film Containing Natural Antimicrobial Material on Cariogenic Bacteria
Kim Ki-Myong

Hwang Kwon-Tack
You Sang-Guan
Lee Ung-Soo
Jung Kyung-Hwan
Moon Sung-Kwon
Choi Won-Seok
Abstract
This study was focused on the development of anticariogenic edible films using pullulan containing grapefruit seed extract (GFSE), polylysine or propolis. According to the result of antimicrobial activity (disc diffusion method) of GFSE, polylysine and propolis against Streptococcus mutans, antimicrobial pullulan film was produced by adding grapefruit seed extract. The optimum combination of pullulan and sorbitol (plasticizer) was 10~15% (w/v) and 40~50% of pullulan (w/w), respectively. Minimum concentration of grapefruit seed extract for growth inhibition of Str. mutans was 50 ppm in medium. Formulation of antimicrobial pullulan films containing grape seed extract was established and these results evidently showed potential for commercial application.
KEYWORD
anticariogenic edible film, grapefruit seed extract, Streptococcus mutans
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